Innovation in label-free biosensing.
Delta Diagnostics’ Technology
Delta Diagnostics develops a true platform technology that can be applied for basic life-science research and in development of new multi-biomarker assays for diagnostics. In the life-science research domain it offers affordable, high-plex analysis (binding kinetics, antibody screening, concentration measurements), while in the diagnostics domain it enables rapid and quantitative detection of panels of biomarkers.
Photonic Integrated Circuits
We make use of integrated photonics, or optical chips. The chips carry wave guides that transport laser light across the chip to many integrated sensors. These sensors are in fact inexpensive, yet extremely sensitive refractive index sensors.
The binding of biomolecules on the sensor surface leads to a local change in refractive index. By functionalising the sensor with for instance an antibody, a highly sensitive and selective biosensor is obtained.
As the individual sensors are only a few hundred µm in size, many sensors fit on a small chip surface.
Each sensor can be functionalised with a different capture molecule, such that many biomarkers can be detected simultaneously even in small and complex samples. Our sensors currently enable 16-plex detection.
An evanescent field extends from the waveguides on the chip surface into the sample and is used to detect changes in refractive index. The refractive index change is proportional to the density of surface-bound biomolecules and can therefore be used to accurately determine the concentration of biomarkers in the sample.
Evanescent field sensing is a label-free technology, meaning that a detection label is not needed. This results in faster assays that require less reagents. In addition, exclusively with label-free technology, binding kinetics can be analysed.
Rapid Assay Development
Label-free detection means that binding of biomolecules can be monitored in real-time. This helps tremendously to limit the time needed to develop assays. For instance, it allows you to rapidly and effectively select the high-affinity antibodies needed for your assays.
Delta Diagnostics successfully developed and validated a small and affordable instrument to read-out its biosensors. A number of industrial and academic partners already rely on our instruments for their research on biomolecular interactions and assay development.